CYTOLOGICAL AND CYTOCHROME C OXIDASE II (COII) GENE CHARACTERIZATION OF THE BEAN PODBORER, Maruca vitrata (Fabricius) (LEPIDOPTERA: CRAMBIDAE)

Maria Luz J. Sison, Maria Luisa D. Guevarra

Abstract


The bean podborer, Maruca vitrata (Fabricius) was characterized through its karyotype and mitochondrial cytochrome c oxidase II (COII) gene.  The chromosomes are irregular in shape, with number ranging from 23 to 66, relative lengths of 0.010 to 0.073 and with no definite centromeric sites.  The partial nucleotide sequence of the COII gene is 662 base pairs that encodes 199 amino acids.  This was submitted to GenBank as Maruca testulalis (Geyer), synonym of M. vitrata, and was assigned Accession No. AY899212.1. The putative initiation codon was ATG at position 64 to 66 and encodes the amino acid methionine.  The gene has high A-T content of 81% with A-T bias in codon usage that preferred the third position.  The alignment of the COII gene with those of other members of family Crambidae that are available in GenBank, revealed that it is most related to Heliothis virescens.

Key words: cytochrome c oxidase II, COII, karyotype, Maruca vitrata


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