IDENTIFICATION OF FLAVONOIDS AND PHENOLIC COMPOUNDS IN PROPOLIS FROM STINGLESS BEE (Tetragonula biroi Friese) NESTS AND EXTRACTS FROM FIVE TREE SOURCES USING TANDEM LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

Paul Lloydson J. Alvarez, Meryl B. Cruz, Jose Rene L. Micor, Alejandro C. Fajardo, Jr, Cleofas R. Cervancia, and Amelia B. Hizon-Fradejas

Abstract


Propolis collected from the nests of stingless bee, Tetragonula biroi, and exudates from five tree species observed to be foraged by T. biroi, namely, mango (Mangifera indica), jackfruit (Artocarpus heterophyllus), pili (Canarium ovatum), caimito (Chrysophyllum cainito) and chico (Achras sapota) were processed to identify the flavonoids and phenolic compounds using tandem liquid chromatography-mass spectrometry (LC-MS). The flavonoids and phenolic compounds identified from the propolis and exudates from the five plant species were similar. They were pinobanksin- 5,7-dimethyl ether and Artepillin C, luteolin-5-methyl ether, pinobanskin-3-0 (butyrate or isobutyrate) and kaempferide. Characterization of each compound present in propolis is necessary to determine its plant origin and biological activity.

Keywords: Tetragonula biroi, propolis, flavonoids, phenolic compounds, LC-MS

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