DNA BARCODING AND MORPHOMETRIC ANALYSES OF ORIENTAL FRUIT FLY SPECIES Bactrocera occipitalis (Bezzi) AND B. philippinensis (Drew and Hancock) (Diptera: Tephritidae) FROM GUIMARAS, PHILIPPINES

Angelo Augusto M. Sumalde, Merlyn S. Mendioro, Michael Leonardo C. Delomen, and Celia dR. Medina

Abstract


DNA and morphometric analyses were done to compare variations between the oriental fruit fly species Bactrocera occipitalis and B. philippinensis. Two base pair sequences (556- and 619 base pairs) found at the 5' end of the mitochondrial cytochrome c oxidase I gene were used. Sequence alignment for both primer sets indicated high sequence similarity (96-100% homology). Construction of neighbor-joining (NJ) phylogenetic trees showed that genetic distance ranged from 0 to 0.161% for the 556 basepair and 0 to 0.014% for the 619 base pair sequences. Morphometric analyses of the scutellum length and width, lengths of femur, tibia, tarsi of fore, middle and hindlegs, antennal segments, and distances between lateral and medial ocelli showed no significant differences between B. occipitalis and B. philippinensis. Based on these results, B. occipitalis could not be delineated from B. philippinensis.

Key Words: Bactrocera occipitalis, B. philippinensis, DNA barcoding, Morphometric analysis

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