Concentration of Hemoglobin Plus Carbonic Anhydrase Isoenzyme-I in the Feces of Rats with Indomethacin-induced Gastric Bleeding
Abstract
A rabbit antibody against rat hemoglobin (Hb) containing carbonic anhydrase isoenzyme I (CA-I) was used. The concentration of Hb plus CA-I (composite measurement) in feces of rats was measured in which gastric bleeding was induced by administering indomethacin at 5, 10 or 35 mg/kg. Concentrations of Hb plus CA-I were measured by enzyme linked immunosorbent assay (ELISA). The concentrations of Hb plus CA-I in aging rats were significantly lower than in younger rats. The concentrations of Hb plus CA-I were increased in feces collected from rats administered 10 or 35 mg/kg indomethacin. The peak concentrations at 48 hr after administration of indomethacin were 17.1 mg/g for 10 mg/kg and 5.91 mg/g for 35 mg/kg; these values were significantly greater than those in the control group. The concentrations of Hb plus CA-I in feces did not increase at 5 mg/kg indomethacin, or in the control group. The measurement of Hb plus CA-I in feces could detect gastric bleeding in rats until it is possible to specifically measure Hb concentration in feces.
Key words: CA-I, feces, gastric bleeding, hemoglobin, indomethacin, rat
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